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Abstract
Keywords
Abstract
BACKGROUND: Vitamin D3 is a growth factor
improving bone mineralization, and regulating
osteoblastic activity and longitudinal bone
growth. In this study, impact of vitamin D3
supplementation administered prior to
fractionated RT in reducing RT-induced
epiphyseal injury is investigated..
MATERİAL-METHOD: Six week old male
Sprague-Dawley rats were enrolled to one of
the four groups: Group 1 was assigned as
control group (n=7); Group 2 received
fractionated RT alone; Group 3 received
50000 IU/kg i.m. vitamin D3 injection alone;
and Group 4 received 50000 IU/kg i.m. vitamin
D3 injection prior to fractionated RT.
Fractionated RT in the irradiated groups was
delivered to distal femur and proximal tibia in
the left legs of each rats to a total dose of 24
Gy in 3 fractions with the contralateral right leg
as the nonirradiated control. Vitamin D3
injection in Group 3 was performed on the day
before the RT. Bone growth was calculated
according to the lengths of femur, tibia and total leg measured on the radiographs taken at
the time and 6 weeks after the delivery of RT.
RESULTS: RT resulted in a mean percent
overall limb growth loss of 56.2% ± 6.7% and
a mean percent overall limb discrepancy of
12.7% ± 1.3%. Administration of 50000 IU/kg
i.m. vitamin D3 before RT reduced the mean
percent overall limb growth loss and the mean
percent overall limb discrepancy to 28.5% ±
5.6% and 4.4% ± 3.3%, respectively. These
values were significantly different compared
with the groups receiving irradiation alone
(P=0.001 for each).
CONCLUSION: In conclusion, these result
demonstrated the potential for vitamin D3
administered before fractionated RT to
significantly reduce the RT-induced epiphyseal
injury.